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. 2016 Aug 24;7:1329. doi: 10.3389/fmicb.2016.01329

Figure 4.

Figure 4

Effects of 2b and its mutants on the virulence and accumulation of viral RNAs in infection with wild-type Arabidopsis plants. (A) Disease symptoms in wild-type Arabidopsis plants inoculated with CMV and CMV2b(x) at 9 dpi and 21 dpi. (B) RNA gel blot detection of viral genomic RNAs and vsiRNAs. Methylene blue-stained ribosome rRNA and U6 were used as the loading control. (C) GFP fluorescence in the leaves of Nb plants coinfiltrated with GFP and 2b or mutants. Coinfiltration of GFP with vector was used as control.