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Effects of 2b and its mutants on the virulence and accumulation of viral RNAs in infection of rdr1/6 plants. (A) Disease symptoms in rdr1/6 plants inoculated with CMV and CMV2b(x) at 21 dpi. (B) RNA gel blot detection of viral genomic RNAs and vsiRNAs. tasiR255 was used to verify the RDR mutant alleles. Methylene blue-stained ribosome rRNA and U6 as well as miR159 were used as the loading controls.
