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. 2016 Aug 24;7:299. doi: 10.3389/fimmu.2016.00299

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Copyright © 2016 Pradier, Simonetta, Waldvogel, Bosshard, Tiercy and Roosnek.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gating strategies for KIR subset analysis. (A) Lymphocyte gates on FSC and SSC. (B) NK cell defined as CD56^posCD3^neg lymphocytes. (C) CD56^bright and CD56^dim NK cells according to the level of CD56-expression and gate on CD56^dimCD94^neg NK cells used for further analysis. (D–F) CD56^dimCD94^neg cells according to KIR expression (eight subsets). Q1: KIR2DL1^negKIR2DL3^negKIR3DL1^pos, Q2: KIR2DL1^posKIR2DL3^negKIR3DL1^pos, Q3: KIR2DL1^negKIR2DL3^negKIR3DL1^neg, Q4: KIR2DL1^posKIR2DL3^negKIR3DL1^neg, Q5: KIR2DL1^negKIR2DL3^posKIR3DL1^pos, Q6: KIR2DL1^posKIR2DL3^posKIR3DL1^pos, Q7: KIR2DL1^negKIR2DL3^posKIR3DL1^neg, Q8: KIR2DL1^posKIR2DL3^posKIR3DL1^neg. Examples of fluorescence intensity of CD57 (G), Eomes (H) and T-bet (I) according to the number of KIR expressed with their respective negative controls.