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siRNA knockdown of PI3K p110δ prevents carbachol‐induced (10 min, 10 μM) MLC phosphorylation (pMLC) by suppression of ROCK in HASM cells. (A) Immunoblot analyses of HASM cells transfected with PI3K p110δ siRNA or scrambled siRNA. (B) Inhibition of carbachol‐induced phosphorylation of MYPT1 (pMYPT1) and MLC (pMLC) in HASM cells by PI3K p110δ siRNA. Data are representative of five independent experiments (n = 5, mean + SEM); *P < 0.05.
