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. 2016 Aug 11;173(18):2780–2792. doi: 10.1111/bph.13556

Figure 2.

Figure 2

Effect of 5A peptide on intestinal inflammation in DSS‐induced colitis in mice. C57BL/6 on DSS (3% w v‐1) or control mice receiving drinking water without DSS were administered, daily, 5A peptide (0.25 mg·mL−1) or PBS by i.p. injection for 10 days. Numbers of animals analysed are provided in graphs. The data shown are pooled from two independent experiments in the colitis group and one experiment in the control group. Data were analysed using one‐way ANOVA followed by Student–Neuman–Keul or Welch's test followed by Games‐Howell post hoc test or Kruskal–Wallis test. (A) Immunohistochemical visualization of mucosa neutrophil infiltrations. Shown are representative images of anti‐Gr‐1 staining in colitic animals. Bar graph: cell count/view was determined as described in Methods. (B) MPO concentration in colonic tissue of 5A peptide‐ or PBS‐treated mice. (C and D) Levels of pro‐inflammatory cytokines IL‐6 (C) and TNFα (D) as determined in plasma from 5A peptide‐ or PBS‐treated mice.