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. 2016 Aug 24;36(34):8790–8801. doi: 10.1523/JNEUROSCI.0168-16.2016

Figure 1.

Figure 1.

BDNF secretion from HD astrocytes is reduced. A, ELISA assay showed that cultured primary astrocytes from TG (Student's t test, n = 6 independent experiments, p = 1.6381E-05) and KI (Student's t test, n = 4 independent experiments, p = 0.0208) mice release less BDNF than astrocytes from WT mice. ELISA results also indicated that BDNF secretion is decreased from brain slices of TG mice compared with WT mice (Student's t test, n = 3 independent experiments, p = 0.0449). However, BDNF secretion is unchanged from brain slices of N171-82Q mice compared with WT mice (Student's t test, n = 3 independent experiments, p = 0.1489). B, WT neurons were treated with ACM containing released BDNF from WT or KI astrocytes, and then analyzed via Western blotting to examine p-TrkB levels. p-TrkB is decreased in KI ACM-treated neurons, confirming decreased BDNF in KI ACM. C, Glutamate measurement assay showing no significant reduction in glutamate release in cultured astrocytes from TG mice (Student's t test, n = 5 independent experiments, p = 0.1027) or KI mice (Student's t test, n = 6 independent experiments, p = 0.3647) compared with WT astrocytes. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.