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. 2016 Aug 24;36(34):8790–8801. doi: 10.1523/JNEUROSCI.0168-16.2016

Figure 2.

Figure 2.

Transcription or translation of BDNF is not changed in the HD astrocytes. A, B, qRT-PCR results revealing no significant reduction in BDNF mRNA levels in cultured astrocytes from TG mice (A, Student's t test, n = 5 independent experiments; BDNF transcripts generated by different promoters are indicated: BDNF promoter I, p = 0.5844; BDNF promoter II, p = 0.3669; BDNF promoter III, p = 0.5765; BDNF promoter IV, p = 0.67; BDNF promoter pan, p = 0.2406) or KI mice (B, Student's t test, n ≥ 5 independent experiments; BDNF promoter I, p = 0.0688; BDNF promoter II, p = 0.2283; BDNF promoter III, p = 0.6892; BDNF promoter IV, p = 0.0809; BDNF promoter pan, p = 0.7733) compared with WT astrocytes. C, D, Western blotting revealing similar mature mBDNF and precursor of BDNF (pro-BDNF) levels in cultured astrocytes from TG (C), KI (D), and WT mice. Quantifying ratios of pro-BDNF or mBDNF to actin in TG astrocytes (C, Student's t test; pro-BDNF, n = 8 independent experiments, p = 0.6983; mBDNF, n = 8 independent experiments, p = 0.4087) and KI astrocytes (D, Student's t test; pro-BDNF, n = 8 independent experiments, p = 0.8801; mBDNF, n = 5 independent experiments, p = 0.6349). ns, Not significant.