Figure 5.

DHA potentiates chimeric channels that include the NMDA receptor TMD and CTD. A, Exposure to 15 μm DHA (shaded bar) potentiated whole-cell current evoked by 10 μm kainate (open bars) in an HEK cell transfected with the N1/N2B TMD+CTD chimera. B, Lack of effect of DHA on the N1/N2B TMD chimera. C, Plot of current (mean ± SEM) evoked immediately after exposure to DHA as a fraction of control current before DHA treatment. *, Significant potentiation for chimeric constructs with the NMDA receptor TMD+CTD, but not for chimeras that included the NMDA receptor TMD and linkers without the CTD (p < 0.05, Mann–Whitney rank-sum tests).