Figure 4.

Phosphorylation of synaptojanin at S1029 is required for normal synaptic vesicle endocytosis. A, Representative images of the NMJs after FM1-43 loading and unloading for the indicated genotypes in synj¹/synj² background. The 60 mm K⁺ stimulation for 5 min was used to load the dye, and 1 min stimulation with 60 mm K⁺ was used to unload FM1-43. Scale bar, 5 μm. B, Quantification of relative load and unload intensity for FM1-43 dye with normalization to loading intensity of the control. C, Quantification of FM1-43 signal removed during unloading normalized to amount of FM1-43 loading. n ≥ 7 NMJ from n ≥ 7 larvae per genotype. Data are mean ± SEM. *p < 0.05 compared with control of the same condition.