Figure 9.

Regulation of ATP-evoked mIPSC facilitation onto POMC neurons and tdTomato expression in AgRP-NPY neurons by metabolic state. A, Representative traces of mIPSCs before and during ATP applications recorded from POMC neurons in normally fed mice, fasted mice, and fasted mice injected with leptin (1 μg/g) 3 h before the experiments. B, Summary of mIPSC frequency and amplitude from experiments, such as those in A. C, Representative tdTomato IHC images for Arc from normally fed mice, fasted mice, and fasted mice injected with leptin. Dotted lines indicate the area in which tdTomato intensity was measured. D, E, tdTomato intensity in neurons (D) and tanycytes (C) from experiments, such as those shown in C.