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. 2016 Aug 10;2016:6769032. doi: 10.1155/2016/6769032

Figure 2.

Figure 2

Expression levels of AKT, eNOS, VCAM-1, and ICAM-1 were modulated by lentivirus-based ALOX15 overexpression in rat balloon-injured (BI) carotid arteries 14 days after transfection. (a) Immunohistochemical analysis of eNOS, VCAM-1, and ICAM-1 in BI carotid arteries. The arrows indicate positive staining. The scale bar represents 5 μm. (b) Western blot analysis of eNOS expression in BI carotid arteries. (c) Western blot analysis of p-AKT/p-eNOS expression in BI carotid arteries to assess the activity levels of AKT and eNOS. The protein level of p-eNOS was normalized against that of GAPDH because total eNOS expression was changed. (d) Western blot analysis of VCAM-1/ICAM-1 expression in BI carotid arteries. The scale bar represents 10 μm. Results are expressed as mean ± SD. Ator: atorvastatin; NS: not significant. ∗ P < 0.01 versus the Sham group; # P < 0.001 and ## P < 0.05 versus the BI group; † P < 0.01 and †† P < 0.05 versus the BI + Lv-GFP group. N = 4 per group.