Figure 2. Changes in phosphorylated (Top) and total (Bottom) ACC protein expression in skeletal muscle following 5 hr light- (5h LI, n = 7) or moderate-intensity (5h MI, n = 7) interruptions, and 3 days of light-intensity interruptions (3d LI, n = 5) compared to uninterrupted sitting (value of 1).

Data represent mean ± SEM. Representative blots are shown above the graphs. Dotted lines are showing where the images have been cropped; Full length blots/gels are presented in Supplemental Figures 1 (IDLE, ACC), 3 (IDLE, Tubulin), 4 (ABLE, ACC) and 5 (ABLE, Tubulin). Protein expression of ACC (280 kDa) and tubulin (52 kDa) have been measured on the same blot. All the blots have been run under exact same conditions. *P < 0.05 vs. sitting.