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. 2016 Aug 24;6:32073. doi: 10.1038/srep32073

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Copyright © 2016, The Author(s)

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(A) Dilution series of wild-type and mutant yeast strains transformed with OsMTP8.1 or the empty vector were spotted onto plates supplemented with metals as indicated. (B) Vacuolar membrane localization of OsMTP8.1. C-terminal GFP fusion protein expressed in the S. cerevisiae strain Δpmr1. Cells are visualized 24 h after transformation. From left to right, shown are GFP fluorescence, bright-field, and merged images with GFP in green. Scale bar 5 μm. (C) Transient expression of OsMTP8.1 in rice mesophyll protoplasts. Cells were visualized at 24 h after transformation. From left to right, shown are GFP fluorescence from OsMTP8.1-GFP, bright-field, and a merged image. Scale bar 5 μm.