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. 2016 Aug 24;6:32073. doi: 10.1038/srep32073

Figure 2. Amino acid sequence alignment of OsMTP8.1 with EcYiiP and other CDF family proteins.

Figure 2

TtCzrB (GenBank Acc. No. AJ307316.1) from Thermus thermophilus, AtMTP1 (GenBank Acc. No. At2g46800), AtMTP8 (GenBank Acc. No. At3g58060) and AtMTP11 (GenBank Acc. No. At2g39450) from Arabidopsis thaliana, CsMTP8 (GenBank Acc. No. AFJ24703) from Cucumis sativus, HvMTP8.1 (GenBank Acc. No. AFP33387) from Hordeum vulgare, PtMTP11.1 (Phytozome: POPTR_0010s21810) and PtMTP11.2 (Phytozome: POPTR_0008s04940) from Populus trichocarpa, OsMTP9 (Phytozome: LOC_Os01g03914) and OsMTP8.1 (Phytozome: LOC_Os03g12530) from Oryza sativa, and E. coli K12 YiiP are aligned. The sequence of AtMTP1 lacks the histidine-rich loop (D185 to K250). The transmembrane helices (TM), external (vacuolar) loops (EL), intracellular (cytosolic) loops (IL), and C-terminal cytoplasmic a-helices and b-sheets of EcYiiP are shown above the alignment. Residues essential for Mn binding in site A and site C of OsMTP8.1 are shown in red. Point denotes a gap in the alignment.