Figure 2.

(a)Top: 8% SDS gel showing band with varying intensity depending on the amount loaded in the well (1000, 500, 250, 100, 50, 25 ng). Bottom: Relationship between the band intensity (au) and known amount of HMM. Bars indicate standard error. Linear fit is with slope 32.66 ± 3.68 and intercept set at zero. (b) Top: 8% SDS gel with eluate from incubated HMM concentration of 500, 250, 100, 50 μg/ml from surface density experiments. Bottom: Relationship between applied HMM concentration (μg/ml) and HMM molecules/μm². The values for the number of HMM molecules/μm² were calculated from the standard plot. Linear fit is for points between 50–400 μg/ml with slope 6.21 ± 0.47 and intercept set at zero.