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. 2016 Aug 24;6:32043. doi: 10.1038/srep32043

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Copyright © 2016, The Author(s)

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(a)Actin filaments before addition of ATP in IVMA. Most of the filaments are >5 μm. (b,c) Snapshot of IVMA at 25 μg/ml and 1000 μg/ml HMM density at 30 °C, 2 mM ATP. Scale bar corresponds to 5 μm. (d) Average length of the actin filaments sliding at different HMM density in an IVMA. (e) Actin sliding velocity (V_F) as a function of HMM density. Actin sliding velocity was 5.5 ± 1.1 μm/s over the entire range of HMM density. Error bars denote standard deviation. All the data points in (d,e) correspond to a single IVMA experiment averaged from a minimum of 30 filaments satisfying our steady sliding criterion.