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. 2016 Aug 24;6:32043. doi: 10.1038/srep32043

Figure 4.

Figure 4

Snapshot of IVMA experiments at (a) 0.05 mM and (b) 2 mM ATP concentration at 100 μg/ml HMM density, 30 °C. At 2 mM ATP concentration, the average filaments length was 1747.6 ± 614.4 nm, and as ATP concentration was reduced to 0.05 mM the average length of filaments reduced to 698.8 ± 114.7 nm. Scale bar corresponds to 5 μm. (c) Length of actin filaments as a function of ATP at HMM density: 100 μg/ml (circles) and 600 μg/ml (diamonds). Bars indicate mean standard deviation from a minimum of three IVMA experiments each including at least 30 filaments.