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. 2016 Aug 24;6:32085. doi: 10.1038/srep32085

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Two-month-old male rats underwent sham-operation (Sham) or ORX for 7 days and the ORX rats were then treated with sesame oil or TP for another 7 days. (A) SMP30 protein expression in rat kidney and liver were analyzed by Western blotting, normalized to β-actin expression in the kidney (B) and liver (C). (D) SMP30 mRNA expression in rat kidney and liver were analyzed by RT-PCR, normalized to gapgh expression in rat kidney (E) and liver (F). Rat plasma testosterone (G) and LH (H) were measured by RIA. Data represent means ± SEM (n = 8). *P < 0.05, **P < 0.01 as compared with the sham-operated rats; ^#P < 0.05, ^##P < 0.01 as compared with the ORX rats. The gels have been run under the same experimental conditions and cropped blots/gels were shown. The entire of membrane/gels pictures of Fig. 2A,D were presented in the Supplementary Figs S4 and S5.