Figure 3. Renal SMP30 expression is androgen-dependent.

(A) Fluorescence microscopy of double labeled two-month-old male rat kidney sections using goat anti-SMP30 (green) and rabbit anti-AR (red) antibodies. DAPI (blue) was used to counterstain the nuclei. Overlay was presented. (magnification 400x; scale bar = 50 μm). g, glomerulus. Two weeks of orchidectomized (ORX) and TP, TP plus flutamide (Flu), and Flu replaced rats were scarified. Kidney (B) and liver (C) tissues were harvested for Western blot of SMP30 normalized to β-actin expression. (D) RT-PCR of SMP30 mRNA expression was performed in the kidney, normalized to gapdh expression. (E) Plasma testosterone was measured by RIA. Data represent means ± SEM (n = 7–8 each). **P < 0.01 as compared with the sham-operated rats; ^##P < 0.01 as compared with the ORX rats; ⁺⁺P < 0.01 as compared with the ORX + TP rats. The gels have been run under the same experimental conditions and cropped blots/gels were shown. The entire of membrane/gels pictures of Fig. 3B–D were presented in the Supplementary Figs S6 and S7.