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. 2016 Aug 24;6:32085. doi: 10.1038/srep32085

Figure 4. Aging effects on SMP30 expression in rat kidney.

Figure 4

Male rats were divided into four groups: young (2–3 months), adult (5–6 months), middle-aged (12–15 months), and old (20–24 month). (A) SMP30 protein expression in rat kidney (n = 9) was analyzed by Western blot, normalized to β-actin expression in the kidney. (B) SMP30 mRNA expression in rat kidney (n = 8) was analyzed by RT-PCR, normalized to gapdh expression in the kidney. (C) Plasma testosterone levels were measured by RIA (n = 9). (D) Pearson correlation coefficients were used to evaluate the relationship between plasma testosterone and renal SMP30 protein expression (n = 36; Pearson: r = 0.601, P < 0.01). Data represent means ± SEM. **P < 0.01 as compared with the young rats; #P < 0.05, ##P < 0.01 as compared with the adult rats; +P < 0.05 as compared with the middle-aged rats. The gels have been run under the same experimental conditions and cropped blots/gels were shown. The entire of membrane/gels pictures of Fig. 4A,B were presented in the Supplementary Fig. S8.