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. 2016 Aug 24;6:32085. doi: 10.1038/srep32085

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Gender difference in protein levels of SMP30 in rat kidney. The renal SMP30 protein levels in two-month-old of both female (n = 8) and male (n = 6) rats were analyzed by Western blot, normalized to β-actin expression. Two-month-old female rats underwent sham-operation (Sham) or OVX for 7 days and the OVX rats were then treated with sesame oil or TP for another 7 days. (B) Kidney (n = 6) and liver (n = 6) tissues were harvested for Western blot of SMP30, normalized to β-actin expression in the kidney (C) and liver (D). Plasma testosterone (E) and estradiol (F) levels were measured by RIA (n = 6). Data represent means ± SEM. **P < 0.01 as compared with female rats; ⁺P < 0.05, ⁺⁺P < 0.01 as compared with sham rats; ^##P < 0.01 as compared with the OVX rats. The gels have been run under the same experimental conditions and cropped blots were shown. The entire of membrane pictures of Fig. 5A,B were presented in the Supplementary Fig. S9.