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. 2016 Aug 24;6:32085. doi: 10.1038/srep32085

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Kidney tissues were harvested from two-month-old male rats. Tissues were formalin-fixed and paraffin-embedded. Four micrometer cross sections of the tissues were collected onto slides. Fluorescence immunohistochemical staining of kidney sections with goat anti-SMP30, mouse anti-aquaporin-1 (AQP1, renal proximal tubule marker protein), rabbit anti-Tamm-Horsfall glycoprotein (THP, the thick ascending limb of the loop of Henle marker protein), and rabbit anti-calbindin-D (renal distal tubule marker protein) antibodies were used to evaluate SMP30 localization. Nuclei counterstaining with 4,6-diamidino-2-phenylindole (DAPI) was used. Overlay was presented. (magnification 400x; scale bar = 50 μm). g, glomerulus.