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. 2016 Aug 24;6:32085. doi: 10.1038/srep32085

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Two weeks of orchidectomized (ORX) and TP, TP plus flutamide (Flu), and Flu replaced rats, and (B) aged male rats were housed in metabolic cages for 24 h and urine was collected for Ca²⁺ analysis. Data represent means ± SEM. **P < 0.01 as compared with the sham-operated rats; ^##P < 0.01 as compared with the ORX rats; ⁺⁺P < 0.01 as compared with the ORX + TP rats; ^$P < 0.05 as compared with young rats; ^&P < 0.05 as compared with adult rats.