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. 2016 Aug 24;6:31988. doi: 10.1038/srep31988

Figure 4. The phosphorylation of CjWRKY1 at Tyr 115 affected the transcriptional activity of the CjWRKY1 protein.

Figure 4

(a) Transactivation activity of mutant CjWRKY1 proteins detected by dual-LUC reporter assays. The top panel shows the gene organization of the effector and reporter constructs. The bottom panel indicates the relative activity level standardized using the value of the VC as 1. The values are the average of medians of five independent experiments with three biological transfections. The data are represented as the mean ± s.d., *p < 0.05, **p < 0.01, n.s., not significant, Student’s t-test. (b) Transactivation activity of GAL4DB-fused mutant CjWRKY1 proteins detected as described in (a). The values are the average of medians of three independent experiments with three biological transfections. The data are represented as the mean ± s.d., *p < 0.05, **p < 0.01, Student’s t-test.