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. 2016 Aug 24;6:31992. doi: 10.1038/srep31992

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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geNormPlus analysis shows the calculation of the average expression stability M-value of all candidate reference genes determined by RT-qPCR (A,C). Genes with the highest M-value have the least stable expression, while the genes with the lowest M-value have the most stable expression. The x-axis presents the ranking of reference genes in order of decreasing stability from left to the right. High stability is defined by an M-value of <0.5 as indicated by the dotted line. Chosen reference miRNAs are highlighted by black squares. By analogy NormFinder Stability-values are listed in an ascending order (B,D). Small-nucleolar RNAs (snoRNAs such as RNU48, RNU44 and U6snRNA) are indicated by coloured arrows. snoRNAs are indicated by coloured arrows.