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. 2016 Aug 24;6:31992. doi: 10.1038/srep31992

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Quantitative differences of six significantly different expressed miRNAs are shown in dependence from different normalization factors. Relative expression levels were calculated using the following normalization methods: geNormPlus (miR-26b, miR-195-5p) NormFinder (miR-28-5p, miR-127-3p, miR-181a-5p), global Mean, best two snRNAs (RNU44, RNU48) and all 3 snRNAs (RNU44, RNU48, snRNU6). Four miRNAs (miR-132-3p, miR-146-5p, miR-184, miR-708-5p) demonstrated high consistency throughout different normalization methods. Significant differences are illustrated by horizontal lines as P values < 0.05 of the nonparametric pairwise ranking test (Steel-Dwass test for multiple comparison analysis). Median values, 25/75 percentiles (grey dotted boxes, IgA-GN; white, crescentic IgA-GN; striped, controls) as well as 10/90 percentiles (whiskers) are shown.