Fig. 4.
Behavioural assessment of male and female mice under T4 excess or deprivation. The chimney test was used to a examine muscle strength, tonus, and coordination of movements in male and female mice under euthyroid, hyperthyroid, or hypothyroid conditions. The open field was used to investigate activity and exploratory behaviour. b Total distance travelled was measured to assess activity, and c frequency of rearings was determined to assess exploratory behaviour. The rotarod test was used for an overall assessment of coordination and motor function in male and female mice before the start of treatment (training period) and under sham (d), T4 (e), or LoI/MMI/ClO4 − (f) treatment. Data are presented as mean ± SD, N = 8 animals/sex/treatment; two-way ANOVA followed by the Bonferroni post hoc analysis applied for sex and treatment effects of a–c and unpaired Student’s t test for sex effect of d–f, *p < 0.05, **p < 0.01, ***p < 0.001 represent multiple-testing or t test results