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. 2015 Dec 11;5:18234. doi: 10.1038/srep18234

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Copyright © 2015, Macmillan Publishers Limited

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Sourdoughs were inoculated with L. reuteri LTH2584 and 100-23 (Panels A and B) or with L. reuteri LTH2584 and LTH5448 (Panels C and D) and maintained by continuous back-slopping with 10% inoculum over 10 fermentation cycles with 1 d incubation times. Sourdough microbiota were analysed by differential plate counts (Panels A and C) and results were expressed as log proportion of the individual strains to the total viable cell counts. Sourdoughs were also analysed by qPCR targeting strain-specific sequences and log DNA copy numbers converted to cell counts using the strain-specific calibration curves (Panels B and D). Results were expressed as proportion of the individual strains to the total cell counts. Symbols represent L. reuteri strains LTH2584 (◼), LTH5448 (□) and 100-23 (◯).