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. 2016 Aug 24;9(1):50. doi: 10.1186/s13048-016-0261-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Transmission electron microscopy of human cryopreserved ovarian tissue after long-term storage. A primordial/primary follicle: the oocyte shows a nucleus (n) having finely dispersed chromatin and cytoplasm (oc) rich in organelles; a layer of flattened/cuboidal granulosa cells (gc) surrounding oocyte cytoplasm; B perinuclear aggregates of rounded mitochondria (m) having moderate electron dense matrices, () nuclear pores; C Golgi apparatus; D annulate lamellae consist of membrane-bound cisternae traversed by pore complexes; E granulosa cells with oval shaped nuclei (n); f stromal cells with oval shaped nuclei (n) and slight band of peripherically condensed chromatin (pch). Empty interstitial areas are detectable ()

Inline graphic — Transmission electron microscopy of human cryopreserved ovarian tissue after long-term storage. A primordial/primary follicle: the oocyte shows a nucleus (n) having finely dispersed chromatin and cytoplasm (oc) rich in organelles; a layer of flattened/cuboidal granulosa cells (gc) surrounding oocyte cytoplasm; B perinuclear aggregates of rounded mitochondria (m) having moderate electron dense matrices, () nuclear pores; C Golgi apparatus; D annulate lamellae consist of membrane-bound cisternae traversed by pore complexes; E granulosa cells with oval shaped nuclei (n); f stromal cells with oval shaped nuclei (n) and slight band of peripherically condensed chromatin (pch). Empty interstitial areas are detectable ()