Table 2.
Clustered single and multi-environment QTL detected in TC and TS populations
Traita | Chrom. | QTL Int. (cM) | Support interval | Peak (cM) | Peak interval | LOD | σ2 (%) | Add | σ2 A | σ2 E | h 2 |
---|---|---|---|---|---|---|---|---|---|---|---|
A) Topas/AG-Castle | |||||||||||
W09 S | A01 | 54–67.5 | sR9564 - sN12790 | 59 | sN11665 - sN12790 | 5.20 | 8.32 | 4.61 | |||
H09 S | A01 | 68–69 | sN12790 - sN4638 | 69 | sR8420 - sN4638 | 17.20 | 15.84 | 7.80 | |||
W10 S | A01 | 68–69 | sN12790 - sN4638 | 69 | sR8420 - sN4638 | 24.25 | 29.90 | 9.31 | |||
W10 II | A01 | 68–69 | sN12790 - sN4638 | 69 | sR8420 - sN4638 | 9.08 | 11.52 | 4.90 | |||
MET II | A01 | 69 | sR8420 - sN4638 | 69 | sR8420 - sN4638 | 34.85 | 20.55 | 6.53 | 12.06 | 8.49 | 0.59 |
MET S | A01 | 68.5–69 | sN12790 - sN4638 | 69 | sR8420-sN4638 | 41.47 | 15.84 | 5.79 | 10.80 | 5.04 | 0.68 |
H09 II | A01 | 69–69.5 | sR8420 - sN12176 | 69.5 | sN4638 - sN12176 | 25.71 | 31.77 | 14.01 | |||
CotQTL | A07 | 73.5–74 | sNRA59 - sR12387b | 73.5 | Rlm3 - sR12829 | 72.37 | 72.18 | 23.29 | |||
H09 S | A08 | 34.5–42.5 | sN4513Fa - sNRB88 | 39 | sN4513Fa - sNRG04 | 8.96 | 7.63 | 5.49 | |||
W09 S | A08 | 34.5–42.5 | sN4513Fa - sNRB88 | 39 | sN4513Fa - sNRG04 | 4.45 | 7.05 | 4.32 | |||
MET S | A08 | 37.5–42 | sN4513Fa - sNRB88 | 39 | sN4513Fa - sNRG04 | 13.59 | 5.13 | 3.51 | 3.85 | 1.28 | 0.75 |
H09 II | A08 | 39.5–47.5 | sNRG04 - sN12352a | 41 | sNRG04 - sNRB88 | 7.42 | 7.85 | 7.08 | |||
MET II | A08 | 39.5–46.5 | sNRG04 - sN12352a | 41.5 | sNRG04 - sNRB88 | 10.29 | 5.26 | 3.39 | 3.16 | 2.10 | 0.60 |
W10 S | A08 | 43–50.5 | sNRB88 - sR9433 | 46 | sNRB88 - sN12352a | 8.34 | 9.08 | 5.19 | |||
W09 II | C06b | 21–22 | brPb - 841625 - brPb - 841355 | 21.5 | brPb - 841625 - brPb - 841355 | 3.04 | 5.17 | 3.50 | |||
H09 S | C06b | 23.5–45 | brPb - 841355 - sN12461Ix | 40 | brPb - 841355 - sN12461Ix | 14.59 | 14.08 | 7.42 | |||
CotQTL | C06b | 23.5–45 | brPb - 841355 - sN12461Ix | 40 | brPb - 841355 - sN12461Ix | 4.71 | 9.26 | 7.25 | |||
MET S | C06b | 36.5–45 | brPb - 841355 - sN12461Ix | 42.5 | brPb - 841355 - sN12461Ix | 25.99 | 9.22 | 5.06 | 8.12 | 1.10 | 0.88 |
W10 II | C06b | 23.5–45 | brPb - 841355 - sN12461Ix | 44.5 | brPb - 841355 - sN12461Ix | 5.88 | 7.35 | 3.94 | |||
H09 II | C06b | 23.5–45 | brPb - 841355 - sN12461Ix | 45 | brPb - 841355 - sN12461Ix | 10.44 | 11.07 | 8.34 | |||
W10 S | C06b | 23.5–45 | brPb - 841355 - sN12461Ix | 45 | brPb - 841355 - sN12461Ix | 10.00 | 10.83 | 5.65 | |||
MET II | C06b | 38–45 | brPb - 841355 - sN12461Ix | 45 | brPb - 841355 - sN12461Ix | 16.44 | 7.83 | 4.30 | 5.14 | 2.70 | 0.66 |
B) Topas/AV-Sapphire | |||||||||||
H12 S | A01 | 30.5–39 | sR9228a - sR9555x | 36.5 | sR6202b - sR9555x | 4.3901 | 10.10 | 5.33 | |||
MET S | A01 | 33.5–39 | sR9228a - sR9555x | 36.5 | sR6202b - sR9555x | 13.1741 | 10.07 | 5.04 | 8.71 | 1.36 | 0.86 |
W11 II | A01 | 30.5–39 | sR9228a - sR9555x | 37.5 | sR6202b - sR9555x | 3.4382 | 11.07 | 4.39 | |||
W11 S | A01 | 30.5–39 | sR9228a - sR9555x | 39 | sR6202b - sR9555x | 6.352 | 18.47 | 8.21 | |||
H12 II | A01 | 36.5–40 | sR6202b - sN12176 | 39.5 | sR9555x - sN12176 | 3.3711 | 9.00 | 4.08 | |||
MET II | A01 | 36.5–40 | sR6202b - sN12176 | 40 | sR9555x - sN12176 | 7.8313 | 5.02 | 4.20 | 4.74 | 0.28 | 0.94 |
CotQTL | A07 | 48–56.5 | sN2555Ra - Rlm4 | 56 | sN2555Ra - Rlm4 | 30.80 | 48.92 | 19.16 | |||
W11 II | A09 | 5–19.5 | sS2212 - sR9373 | 8 | sR6410 - sR9373 | 5.35 | 16.38 | 5.41 | |||
H12 II | A09 | 6.5–14.5 | sS2212 - sR9373 | 8 | sR6410 - sR9373 | 3.05 | 8.12 | 3.92 | |||
W11 S | A09 | 5–19.5 | sS2212 - sR9373 | 8 | sR6410 - sR9373 | 5.34 | 15.00 | 7.48 | |||
MET S | A09 | 6.5–12.5 | sS2212 - sR9373 | 8 | sR6410 - sR9373 | 10.41 | 9.18 | 4.67 | 7.33 | 1.86 | 0.80 |
W09 II | A09 | 8–11.5 | sR6410 - sR9373 | 8.5 | sR6410 - sR9373 | 2.89 | 11.83 | 6.68 | |||
MET II | A09 | 8–14 | sR6410 - sR9373 | 9.5 | sR6410 - sR9373 | 13.97 | 9.66 | 5.88 | 9.10 | 0.56 | 0.94 |
H08 II | A09 | 21–29 | sR9373 - sR6966 | 25 | sR9373 - sR6966 | 3.43 | 12.31 | 9.82 | |||
H12 S | A09 | 21.5–33.5 | sR9373 - sR12078a | 27.5 | sR9373 - sR6966 | 3.41 | 9.47 | 5.19 | |||
H08 II | C06 | 38–60 | sS2486a - sN5088F | 52.5 | sS2486a - sR12387a | 4.79 | 19.31 | 12.24 | |||
H12 S | C06 | 35.5–60 | sS2486a - sN5088F | 54.5 | sS2486a - sR12387a | 6.91 | 22.18 | 7.88 | |||
H12 II | C06 | 40.5–60 | sS2486a - sN5088F | 56.5 | sS2486a - sR12387a | 4.55 | 13.63 | 5.00 | |||
MET II | C06 | 50.5–58.5 | sS2486a - sN5088F | 57 | sS2486a - sR12387a | 10.84 | 9.67 | 4.97 | 6.69 | 2.98 | 0.69 |
H08 S | C06 | 43–60 | sS2486a - sN5088F | 58 | sS2486a - sR12387a | 4.58 | 15.26 | 4.73 | |||
MET S | C06 | 53.5–58.5 | sS2486a - sN5088F | 58 | sS2486a - sR12387a | 14.65 | 9.34 | 5.04 | 8.76 | 0.58 | 0.94 |
Significant QTL shown only (Single environment QTL > 5 σ2(%), MET QTL > 5 σ2(%) and h 2 > 0.5)
aSingle environment (plain) and multi-environment (bold) QTL, single-environment trait names given as location (H = Horsham, W = Wagga Wagga), year (08–12 = 2008–2012) and metric (S = survival, II = internal infection), MET = Multi-environment traits (all environments) for S (survival) and MET II (internal infection) metrics, CotQTL = single-isolate cotyledon tests. Chrom. = B. napus chromosome; QTL Int. (cM) = QTL interval (in centiMorgans); Support Interval = map interval, defined by flanking markers, which contains QTL (LOD > significance threshold); Peak (cM) = Position of Peak LOD value (in centiMorgans); Peak Interval = map interval containing QTL peak LOD; LOD = peak logarithm of odds; σ2(%) = variance (total percentage); Add = additive effect (positive score indicates net genetic contribution from AG-Castle or AV-Sapphire parent); σ² A variance (additive) portion (%), σ² E variance (environmental) portion (%), h 2 heritability (σ² A/σ² (%))