Table 7.
Lmx1b regulates the glutamatergic phenotype of a subset of V0v neurons
| slc17a6 (glutamatergic) | EGFP (V0v) | co-labeled | ||||
| 48 h | WT | lmx1bb -/- | WT | lmx1bb -/- | WT | lmx1bb -/- |
| Mean | 155 | 142.2 | 92.3 | 93.4 | 44.3 | 32.4 |
| SEM | 1.54 | 2.22 | 2.72 | 2.58 | 0.9 | 1.9 |
| n | 6 | 5 | 6 | 5 | 6 | 5 |
| p value | <0.001 | 0.76 | <0.001 | |||
| DsRed (glutamatergic) | EGFP (V0v) | co-labeled | ||||
| 7 dpf | WT | lmx1bb -/- | WT | lmx1bb -/- | WT | lmx1bb -/- |
| Mean | 132.6 | 105.3 | 100.1 | 91.3 | 42.9 | 21.8 |
| SEM | 5.5 | 5.2 | 1.4 | 2.1 | 4.9 | 2.7 |
| n | 9 | 12 | 9 | 12 | 9 | 12 |
| p value | <0.005 | 0.002 | <0.001 | |||
Mean number of cells expressing slc17a6, EGFP or both in the spinal cord region adjacent to somites 6–10 in 48 h embryos and the mean number of cells expressing DsRed, EGFP or both in the spinal cord region adjacent to somites 6–10 in 7 dpf embryos. SEM is the standard error of the mean. n is the number of embryos analyzed for each data set. p value is from a student’s paired t-test comparing WT embryos and lmx1bb homozygous mutants. Statistically significant values are indicated in bold