EtrB negatively regulates transcription of fimbrial locus 11 and genes located in the ETT2. (A) qRT-PCR of Z4498 in WT 86-24 and the ΔetrB strain. (B) qRT-PCR of Z4498 in the WT 86-24 strain transformed with an empty vector control, the ΔetrB strain transformed with an empty vector control, and the ΔetrB strain complemented with etrB (Δn = 3; error bars represent the geometric means ± standard deviations). (C) qRT-PCR of indicated genes in WT 86-24 and the ΔetrB strain. Statistical significance is shown relative to results with WT 86-24 unless otherwise indicated. *, P ≤ 0.05; **, P ≤ 0.005; ns, not significant.