TABLE 2.
Primers used in this study
| Primer name | Sequence | Primer use |
|---|---|---|
| etrB_GenComp_F | AAGCTTGTATTCTTTGGATTTTGCTTA | etrB complement |
| etrB_GenComp_R | CCATGGACTAGGCTTAATGAACTAGA | etrB complement |
| etrB_MalComp_F | CCTGCAGGTATAGTGCACACACCCATAC | etrB::MBP fusion |
| etrB_MalComp_R | CCATGGGAATGATGGGGGCCGAACTC | etrB::MBP fusion |
| etrB_Lux_F | GTTTAAACTAATTATATTTCATTATTATTTCATC | etrB::lux fusion |
| etrB_Lux_R | TACGTATTAACCCATTTTACGAGTTC | etrB::lux fusion |
| etrB_LR_F | TATTTTAGGAGAATTTGCAGGTGGAATGATGGGGGCCGAACTCGTAAAATTGTGTAGGCTGGAGCTGCTTC | etrB mutant |
| etrB_LR_R | CAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCACACACCCATACGTCATATGAATATCCTCCTTAG | etrB mutant |
| etrB_RT_F1 | GGGCCGAACTCGTAAAATGG | qRT-PCR |
| etrB_RT_R1 | ATACGCATCCTTTCGCACCT | qRT-PCR |
| ler_RT_F1 | CGACCAGGTCTGCCC | qRT-PCR |
| ler_RT_R1 | GCGCGGAACTCATC | qRT-PCR |
| grlA_RT_F1 | CCGGTTGTTCCAGGACTTTC | qRT-PCR |
| grlA_RT_R1 | TAAGCGCCTTGAGATTTTCATTT | qRT-PCR |
| escC_RT_F1 | GCGTAAACTGGTCCGGTACGT | qRT-PCR |
| escC_RT_R1 | TGCGGGTAGAGCTTTAAAGGCAAT | qRT-PCR |
| escV_RT_F1 | TCGCCCCGTCCATTGA | qRT-PCR |
| escV_RT_R1 | CGCTCCCGAGTGCAAAA | qRT-PCR |
| espA_RT_F1 | TCAGAATCGCAGCCTGAAAA | qRT-PCR |
| espA_RT_R1 | CGAAGGATGAGGTGGTTAAGCT | qRT-PCR |
| eae_RT_F1 | GCTGGCCCTTGGTTTGATCA | qRT-PCR |
| eae_RT_R1 | GCGGAGATGACTTCAGCACTT | qRT-PCR |
| rpoA_RT_F1 | GCGCTCATCTTCTTCCGAAT | qRT-PCR |
| rpoA_RT_R1 | CGCGGTCGTGGTTATCTG | qRT-PCR |
| ygeI_RT_F1 | TAGCGAATGCAACGGGTGAT | qRT-PCR |
| ygeI_RT_R1 | GACGCCATCCATGTTGAAACT | qRT-PCR |
| yqeK_RT_F1 | ATGGACATTGAGTTTTCGCAGA | qRT-PCR |
| yqeK_RT_R1 | CCCATGATGTTGTTTGCGTGA | qRT-PCR |
| ryeA_RT_F1 | AGATGACGACGCCAGGTTTT | qRT-PCR |
| ryeA_RT_R1 | ACCAGAACGGGCGGTTTTTA | qRT-PCR |
| tnaA_RT_F1 | TGTACACCGAGTGCAGAACC | qRT-PCR |
| tnaA_RT_R1 | CCGTCATACAGACCTACGGC | qRT-PCR |
| eivF_RT_F1 | TTGTTTGCTGATGCCTTGCC | qRT-PCR |
| eivF_RT_R1 | CGCTGCTCAGATAAGTGGCT | qRT-PCR |
| etrA_RT_F1 | TGCAAGTCTTTTCCAGTGATGTC | qRT-PCR |
| etrA_RT_R1 | CCAACGCAACTAAATCGCTGT | qRT-PCR |
| malK_RT_F1 | CGCAATCGATCAAGTGCAGG | qRT-PCR |
| malK_RT_R1 | GTCAGCGATATCACTCGGCA | qRT-PCR |
| nleA_RT_F1 | TGTTGAAGGCTGGAAGTTTGTTT | qRT-PCR |
| nleA_RT_R1 | CCGCTACAGGGCGATATGTT | qRT-PCR |
| Z4498_RT_F1 | CTTGGCAAAAGTGGGCTCTT | qRT-PCR |
| Z4498_RT_R1 | CCGCATCGTCAATACGGATA | qRT-PCR |
| stx2a _RT_F1 | ACCCCACCGGGCAGTT | qRT-PCR |
| stx2a _RT_R1 | GGTCAAAACGCGCCTGATA | qRT-PCR |
| etrB_prom_F1 | TATTATTTCATCAATGTATTCTTT | EMSA |
| etrB_prom_R1 | CGATTTAACCCATTTTACGA | EMSA |
| kan_EMSA_F1 | CCGGAATTGCCAGCTGGGGCG | EMSA |
| kan_EMSA_R1 | TCTTGTTCAATCATGCGAAACGATCC | EMSA |
| ler_emsaF | ATGCAATGAGATCTATCTTA | EMSA |
| ler_emsaR | AATATTTTAAGCTATTAGCG | EMSA |
| amp_emsaF | GGAATTCGAAAGGGCCTCGTGATACGC | EMSA |
| amp_emsaR | CGGGATCCGGTGAGCAAAAACAGGAAGG | EMSA |
| Z4175_R | GATAGCATAGGGAAGAACAG | RT-PCR |
| Z4177_F | TCACTGGCTCAGGTTTAATG | RT-PCR |
| Z4178_F | GGAATGTCCATATTGCATATC | RT-PCR |
| Z4176_F | TTACAACTCATAGCTGATGG | RT-PCR |
| Z4176_R | TTCCATACGCATCCTTTCGC | RT-PCR |
| etrB_PE_R | CGACTGTTTTCCTGCTTAAC | RT-PCR |