TABLE 3.
Locations of SNPs in the N. brasiliensis P-200 genomea
| SNP no. | Location | Original codon (amino acid) | Nucleotide change (amino acid) | Gene | Protein designation | Location |
|---|---|---|---|---|---|---|
| 1 | 263186 | CGC (R) | CTC (L) | O3I_001145 | Hypothetical protein | 261412–263706 |
| 2 | 307213 | CAG (Q) | AAG (R) | O3I_001340 | Hypothetical protein | 305875–307494 |
| 3 | 307214 | CAG (Q) | AGG (R) | O3I_001340 | Hypothetical protein | 305875–307494 |
| 4 | 415131 | GCG (A) | ACG (T) | O3I_001810 | Putative trypsin-like serine protease | 414578–415738 |
| 5 | 442835 | ATG (M) | GTG (V) | O3I_001920 | DNA topoisomerase I subunit omega | 441014–443857 |
| 6 | 939436 | GCG (A) | GCA (A) | O3I_004090 | Putative nonribosomal peptide synthetase (modular protein) | 925811–942625 |
| 7 | 1022704 | GCT (A) | GCC (A) | O3I_004395 | Putative peptidase | 1021063–1023102 |
| 8 | 1022963 | GCG (A) | GTG (V) | O3I_004395 | Putative peptidase | 1021063–1023102 |
| 9 | 1214362 | CGG (R) | TGG (W) | O3I_005190 | Homoserine O-acetyltransferase | 1213901–1215061 |
| 10 | 1240877 | GAA (E) | GAT (D) | O3I_005320 | d-Alanyl-d-alanine carboxypeptidase | 1240385–1241674 |
| 11 | 1429121 | GCC (A) | GTC (V) | O3I_006200 | Homoserine kinase | 1428787–1429752 |
| 12 | 1451830 | Intergenic region | ||||
| 13 | 1451835 | Intergenic region | ||||
| 14 | 1451836 | Intergenic region | ||||
| 15 | 2082888 | GTC (V) | TTC (F) | O3I_009135 | O-Dimethylpuromycin-O-methyltransferase | 2082487–2083548 |
| 16 | 2277017 | Intergenic region | ||||
| 17 | 3631212 | Intergenic region | ||||
| 18 | 3631314 | Intergenic region | ||||
| 19 | 3631353 | Intergenic region | ||||
| 20 | 3631428 | Intergenic region | ||||
| 21 | 3631431 | Intergenic region | ||||
| 22 | 3656905 | GTC (V) | GTG (V) | O3I_016400 | FAD-dependent oxidoreductase | 3656411–3657805 |
| 23 | 3864067 | TCC (S) | TTC (F) | O3I_017285 | Acyl-CoA dehydrogenase | 3863076–3864872 |
| 24 | 5105062 | Intergenic region | ||||
| 25 | 6684580 | Intergenic region | ||||
| 26 | 6684588 | Intergenic region | ||||
| 27 | 6777353 | Intergenic region | ||||
| 28 | 7492092 | Intergenic region | ||||
| 29 | 7492093 | Intergenic region | ||||
| 30 | 7794000 | GGC (G) | GGG (G) | O3I_034520 | Membrane-bound C5 sterol desaturase Erg3 | 7793862–7794752 |
| 31 | 7924188 | Intergenic region | ||||
| 32 | 7997064 | GCG (A) | GCA (A) | O3I_035455 | Hypothetical protein | 7996777–7997214 |
| 33 | 8020777 | GAC (D) | AAC (N) | O3I_035540 | Alpha-ketoglutarate decarboxylase | 8019496–8023245 |
| 34 | 8296987 | GAA (E) | TAA (stop) | O3I_036955 | Transcriptional regulator | 8296886–8297533 |
| 35 | 9157861 | TGG (W) | TGA (stop) | O3I_040880 | Hypothetical protein | 9157676–9158764 |
| 36 | 9419279 | AGT (S) | GGT (G) | O3I_042080 | Peptidoglycan lipid II flippase | 9415559–9419329 |
a
Nucleotide positions were determined by aligning P-200 contig sequences against the N. brasiliensis reference sequence (accession no. NC_18681.1).