FIG 1.

Alveolar macrophages are permissive to C. burnetii replication. BMDMs from C57BL/6 and A/J mice (A and B), BMDMs and AMs from C57BL/6 mice (C), or AMs from C57BL/6 and A/J mice (D) were infected with C. burnetii phase II (MOIs, 100 [A] and 3 [B to D]), and bacterial multiplication was evaluated by quantification of the genomic DNA by qPCR after 0 (4 h after infection), 3, 6, 9, 12 or 15 days (d) of infection. The y axis represents the difference in the base 10 logarithm between the genomic quantity X at time t [logX(t)] and the quantity X at time zero (T₀) [logX(0)]. Cells treated with RIF were used as a negative control of bacterial replication. (Insets) Amount of C. burnetii genomic DNA present in cells after 4 h of infection. The data shown are the means ± standard errors of one representative experiment out of three performed. *, P < 0.05 (two-way ANOVA).