FIG 4.

Alveolar macrophages exhibit higher levels of expression of genes related to the M2 phenotype. BMDMs and AMs from C57BL/6 mice were left uninfected or infected for 12 h with C. burnetii at an MOI of 3. Total RNA was extracted, and reverse transcription-PCR (RT-PCR) was performed for detection of the genes Nos2, Tnfa, Ifng, Tlr2, Il4, Arginase1, Fizz1, and Ym1. The data shown are the means ± standard errors from one representative experiment out of two performed. *, P < 0.05 comparing BMDMs and AMs; #, P < 0.05 comparing infected cells with the uninfected cells from the respective group (two-way ANOVA).