FIG 2.

The tryptophan codon content of a gene influences its transcription in C. pneumoniae during IFN-γ-mediated persistence. Nucleic acid samples were collected from untreated (solid symbols) and IFN-γ-treated (open symbols) C. pneumoniae-infected HEp2 cells at 24 and 48 h postinfection. Transcript levels for the indicated genes were measured by RT-qPCR and normalized to genomic DNA (nanograms of cDNA/gDNA). (A) Quantification of transcript levels for genes containing no Trp codons: mreB, dksA, nusA, and rho. (B) Quantification of transcript levels for genes containing various numbers of Trp (W) codons: rodZ (5 codons), ubiA (5 codons), nusG (4 codons), and obg (2 codons). Data are averages from at least 2 experiments with standard deviations displayed. *, P ≤ 0.05 for 48-hpi IFN-γ versus 24-hpi IFN-γ.