FIG 6.

Immortalized mouse airway epithelial cell lines (LA-4) and human cells (A549 cells) were grown in the presence or absence of TNF-α–IL-1β (100 ng/ml) with and without IL-17 at doses ranging from 1 ng/ml to 100 ng/ml. After 24 h, the supernatants were harvested and analyzed for mouse KC (A) or human IL-8 (B). Student's t tests along with nonparametric multivariate analysis to control for the different conditions were done. Black asterisks, comparisons between the different IL-17 concentrations alone; gray asterisks, comparisons between the TNF-α–IL-1β and IL-17 cytokine cocktail. Significance is defined by a P value of ≤0.05 using a two-tailed analysis.