FIG 8.

CF and WT animals were infected with a P. aeruginosa suspension, and lungs were obtained at 24 h postinfection. Whole-lung homogenates were processed for mRNA and cDNA, and samples were validated for the presence of GAPDH prior to array analysis. One microgram of cDNA was evaluated in each array. The IL-17 response was validated and found to be associated with the SOCS pathway and chemokines. Significance was defined by the relative difference between the CF and WT mouse responses at 24 h postinfection with P. aeruginosa. Each square is a gene, and the region of significance is defined by a midline change in the threshold cycle value (DCt) of 1. (A) Transcriptional regulation through SOCS and NF-κB; (B) chemokine regulatory pathways.