FIG 6.

Subdominant parasite-derived epitopes are not recognized by CD8⁺ T cells in infected TSKB20/18 Tg mice. (A, B) Cumulative data from peptide-stimulated splenocytes from naive and acutely (A) or naive and chronically infected (B) WT or TSKB20/18 Tg mice. CD8⁺ CD4⁻ gated events were assessed for IFN-γ staining after 5 h of incubation with 1 μM indicated peptide alone or indicated combined peptide pool. Data are representative of 5 acute-phase experiments at 18 to 21 days postinfection (n = 11 to 17 mice per infected group) and 4 chronic-phase experiments at 238 to 338 days postinfection (n = 11 to 13 mice per infected group). Filled gray bars are uninfected naive mice, filled black bars are WT, and open bars are TSKB20/18 Tg. Data are means +SEM.