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Cytotoxicity of conditioned medium for primary osteoclasts. Primary bone marrow-derived murine osteoclasts were exposed to CM from cultures of the indicated strains. After 12 h, viability was assessed by TRAP staining, with the graph representing the results of quantitative analysis of all replicates. (Inset) TRAP-positive multinucleated cells. *, statistical significance compared to the results observed with the isogenic parent strain.
