FIG 9.

Impact of PSMs on cytotoxicity for primary osteoblasts and osteoclasts. Primary osteoblast cells were exposed to CM from cultures of the indicated strains. Viability was assessed after 24 h using Invitrogen Live calcein-AM staining. (Left) The results of calcein-AM staining are reported as the average mean fluorescence intensity (MFI) ± standard deviation. (Right) Primary bone marrow-derived murine osteoclasts were exposed to CM from cultures of the indicated strains. After 12 h, viability was assessed by TRAP staining, with the graph representing the results of quantitative analysis of all replicates. *, statistical significance compared to the results observed with the isogenic parent strain in both cell types.