TABLE 1.
Kinetic analysis of Gb3 polymer 1:0 binding to a series of Stx2d B-subunit mutant formsa
| Stx2d B subunit | Mutated site | Mean KD (μM) ± SE | Mean RUmax ± SE (n) |
|---|---|---|---|
| 2dBH | 0.32 ± 0.03 | 438 ± 69.3 (8) | |
| 2dBH-N16A | 1 | 58.7 ± 33b | 19.6 ± 6.4c (3) |
| 2dBH-W29A | 1 | 34.5 ± 7.1 | 53.1 ± 2.4d (3) |
| 2dBH-G61A | 2 | 1.53 ± 0.05 | 246 ± 18.6 (3) |
| 2dBH-W33A | 3 | 16.5 ± 5.3 | 60.8 ± 13.3d (3) |
a
The kinetics of Gb3 polymer 1:0 binding to each immobilized Stx2d B-subunit mutant form were analyzed with the Biacore system. RUmax, maximum resonance unit. The concentration of Gb3 polymer 1:0 is shown as the trisaccharide moiety. The resonance unit is an AU used by the Biacore system.
b
P = 0.027 (compared with 2dBH by ANOVA and Scheffe's test).
c
P < 0.01 (compared with 2dBH by ANOVA and Scheffe's test).
d
P < 0.02 (compared with 2dBH by ANOVA and Scheffe's test).