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Internalization of VacA-Δ(346-347)-Str312 by gastric epithelial cells. AGS cells were treated for 4 h in the presence of 5 mM ammonium chloride with 5 μg/ml of either acid-activated WT VacA (top panels) or VacA-Δ(346-347)-Str312 (bottom panels), each labeled with Alexa Fluor 488. The cells were then washed with PBS, fixed with 4% paraformaldehyde, and stained with Hoechst to label DNA. Fluorescence and DIC images are shown. Scale bar, 10 μm.
