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GC-MS of the neutral sugar composition of G-50 column fractions. Shown is the total ion chromatogram of carbohydrate constituents by alditol acetate derivatives on Sephadex G-50 fraction pools; the peak in lines 2 to 7 at retention time 13.5 min is the characteristic peak for 6-O-methylglucose, and that at retention time 15.2 min is for glucose. Both are characteristic constituents of mGLP; fraction PD G50 01 without contaminating mannose (Man) was further used for biological studies.
