TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| B. burgdorferi | ||
| 297 | Infectious, low-passage B. burgdorferi strain | 51 |
| OY08 | 297; ΔbosR::Kan | 37 |
| OY20 | 297 transformed with pOY63 | 53 |
| OY23 | 297 transformed with pOY69 | 53 |
| OY39 | 297 transformed with pOY90 | This study |
| OY44 | 297 transformed with pOY106 | This study |
| OY68 | OY08 transformed with pOY69 | This study |
| OY69 | OY08 transformed with pOY106 | This study |
| OY79 | 297 transformed with pOY89.2 | This study |
| OY81 | OY08 transformed with pOY89.2 | This study |
| OY82 | OY08 transformed with pOY90 | This study |
| OY318 | 297 transformed with pOY463 | This study |
| OY319 | OY08 transformed with pOY463 | This study |
| E. coli | ||
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) f80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Thermo Fisher Scientific |
| Plasmids | ||
| pGEM-Teasy | TA cloning vector | Promega |
| pOY16 | B. burgdorferi flaB cloned into pGEM-T Easy | 27 |
| pPROEX HTb | Cloning vector | Thermo Fisher Scientific |
| pOY21 | bosR (PCR product from primers 26F and 26R) cloned into pPROEX HTb | This study |
| pOY63 | Promoterless lucBb+ from pJD48 cloned into pJD54 at BglII and HindIII sites | 53 |
| pOY69 | pOY63::dbpBA minimal promoter | 53 |
| pOY89.2 | pOY63::UPbb0648 (PCR product from primers 91F and 91.2R) | This study |
| pOY90 | pOY63::UPbosR (PCR product from primers 92F and 92R) | This study |
| pOY106 | pOY63::ospC minimal promoter (PCR product from primers 108F and 108R) | This study |
| pOY463 | pOY63::UPbb0648-bb0648-UPbosR (PCR product from primers 91F and 92R) | This study |