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. 2016 Jul 28;113(33):9375–9380. doi: 10.1073/pnas.1602960113

Fig. 3.

Fig. 3.

SEP2 mediates the function of FHY3 in FM determinacy. (A) The FHY3-FLAG ChIP-seq peaks (two biological replicates) at SEP1 and SEP2 revealed in IGV. FLAG-FHY3 peaks (purple and orange), gene structure, and the regions examined by ChIP are shown in the top, middle, and bottom rows, respectively. (Scale bars, 500 bp.) (B) ChIP to measure FHY3 occupancy at SEP1 and SEP2 in 35S:3FLAG-FHY3-3HA fhy3-4 inflorescences. The regions examined are shown in A. eIF4A1 served as a negative control. Error bars represent SD from three biological replicates. **P < 0.01 compared with no antibody (negative control). (C) The transcript levels of SEP1 and SEP2 in FHY3:FHY3-GR fhy3-4 inflorescences measured by RT-qPCR. Ubiquitin 5 (UBQ5) served as the internal control. Three biological replicates were performed. Error bars represent SD from three biological repeats. **P < 0.01. (D and E) Siliques from plants of the indicated genotypes. Carpels were indicated by red arrows; Sliced open siliques were indicated by white arrows. (Scale bars, 1 mm.)