Primers for ChIP |
|
SEP1p1F
|
5′-GAGAGCGTGGGAATGAAAGTAAA-3′ |
SEP1p1R
|
5′-AAGAAATATGTGAGGGTGAGGTTAGG-3′ |
SEP1p2F
|
5′-CCTCCTCCCATATGATGACTTCTC-3′ |
SEP1p2R
|
5′-CATCTCTAGTTTTCTTGTTTCTGATTATAGTCT-3′ |
SEP2p1F
|
5′-GGGAGAGAAGGTGTGAGAATGTTT-3′ |
SEP2p1R
|
5′-AGAGATCATTAGGGTTTGGTGATACA-3′ |
SEP2p2F
|
5′-GCCGATCATATCTTCCAGCTAAA-3′ |
SEP2p2R
|
5′-TTCACATTCTTCAACTGCTACATGTT-3′ |
ELF4F
|
5′-CGGACACCGAGGCGAGTA-3′ |
ELF4R
|
5′-ACGCGCGTGTTCTTTGATATC-3′ |
WUSp1F
|
5′-AGATCCGATCACTGAAGTTGCTT-3′ |
WUSp1R
|
5′-TTTGCACGTTGTGTTGAATCC-3′ |
WUSp2F
|
5′-GCACATTTTTCAATAGGGTTT-3′ |
WUSp2R
|
5′-AGGTTTTGTGTGAGAGAGAAGA-3′ |
WUSp3F
|
5′-TTGTAATGGTTTGCTATTGTAC-3′ |
WUSp3R
|
5′-GTTGATTTTGGTCGTTATCC-3′ |
CLV3p1F
|
5′-CCCCTTCTCATTTCATTACCA-3′ |
CLV3p1R
|
5′-GCAGAAAACTCTTCGAATCCA-3′ |
CLV3p2F
|
5′-ATTAGAGTATGTGCCGGTGCC-3′ |
CLV3p2R
|
5′-TGCTGTGGAGGTTCACAACTAA-3′ |
CLV3p3F
|
5′-CAAGGCTCATATAATCCATTCA-3′ |
CLV3p3R
|
5′-CAATATGGATGATACCTTAATCGG-3′ |
CLV3p4F
|
5′-AAGACCTAATAAATTCGTGGACTTG-3′ |
CLV3p4R
|
5′-GAACAATTTTAAAGACATTCGGACC-3′ |
AT3G01520 F
|
5′-GCGAAGGGTTCTTTGTCTTCT-3′ |
AT3G01520 R
|
5′-CACCATCACTTTTGTTGGCTC-3′ |
AT3G03450 F
|
5′-CAAACAATAGTGGCATGCTCTG-3′ |
AT3G03450 R
|
5′-AAAGGAGATTCTGAGAGGAGCA-3′ |
AT5G47800 F
|
5′-CATCGACCCACGTTGACTACT-3′ |
AT5G47800 R
|
5′-TGTTTATCCCCACGAATCTCA-3′ |
AT4G02630 F
|
5′-ATGATGAGTGCAAGTCCCATC-3′ |
AT4G02630 R
|
5′-TTGCTTTTGTTCTGCTTCACA-3′ |
AT5G23940 F
|
5′-GGGAAGTAGAGTCCTTGAAATCC-3′ |
AT5G23940 R
|
5′-GGATTTGATGAAGTTGTTGGG-3′ |
AT3G15820 F
|
5′-AAAGGTAAAGCCCACCAAAGA-3′ |
AT3G15820 R
|
5′-ATACAAGGCGGAGATTTCCAA-3′ |
AT5G51430 F
|
5′-ACTGCGTGGTCTCCAAGTAAA-3′ |
AT5G51430 R
|
5′-AACCAAACCAAAGTAAACGGAA-3′ |
AT4G03330 F
|
5′-CATGGTACCAACCAAATCACA-3′ |
AT4G03330 R
|
5′-TTAAGAGCCGTCTGAAACCAA-3′ |
AT5G19875 F
|
5′-AACACCCATTCAAAGCATACTC-3′ |
AT5G19875 R
|
5′-CCTTCTTCTTGGTCTTCTCTGG-3′ |
AT5G67200 F
|
5′-TCACAGCTGCTTCCCATATTT-3′ |
AT5G67200 R
|
5′-GGAGAATGAAGAAGAAGAAGAAGG-3′ |
eIF4A1F
|
5′-GCTGAGTTGGGAGATCGAAGTT-3′ |
eIF4A1R
|
5′–TCTGGTTCCTCCCGTGTTCT-3′ |
Primers for real-time PCR |
|
SEP1qPCRF
|
5′-CAAGCAAGTTCGGTCCATCA-3′ |
SEP1qPCRR
|
5′-TGCCAAAGCTCTATTGGTTTCA-3′ |
SEP2qPCRF
|
5′-TGCAAGGTCAGTCCCAACAA-3′ |
SEP2qPCRR
|
5′-CCCCACCAGTACTTGCTTAAGATC-3′ |
SEP3qPCRF
|
5′-TGACCAGCTCAACGATCTTCA-3′ |
SEP3qPCRR
|
5′-GCTGGAGTGGCATCTGATACC-3′ |
SEP4qPCRF
|
5′-TGTCCTTTGTGATGCTGAGATTG-3′ |
SEP4qPCRR
|
5′-ATCAACCGTCCTCGCCATAC-3′ |
FHY3qPCRF
|
5′-TTTTGAAAACAACCAAGACCCAATGGTAACAT-3′ |
FHY3qPCRR
|
5′-GCGAGGGGATGGAAGAAAGATGAC-3′ |
CLV3qPCRF
|
5′-ATGGAGAAGCAGAGAAGGCAAA-3′ |
CLV3qPCRR
|
5′-GGGTTCACATGATGGTGCAA-3′ |
FHY1qPCRF
|
5′-GATGAAAGAGGAATCATCTGGA-3′ |
FHY1qPCRR
|
5′-AATCCTCTAAGTTCTGAGTCCCA-3′ |
AGqPCRF
|
5′-CTCAGGAACTTGGAAGGCAGAT-3′ |
AGqPCRR
|
5′-CTCTTTTCTGCATGTAGTCGATTTCA-3′ |
WUSqPCRF
|
5′-GCATCGCCACCACATTCTT-3′ |
WUSqPCRR
|
5′-TGGGCAAACATGGATCATCA-3′ |
CYCB1.3 F
|
5′-TAGCTGAGTTGGGTTTGATGC-3′ |
CYCB1.3 R
|
5′-GCCTGTGTGGAATTTCAGTGT-3′ |
MCM10 F
|
5′-CCAGACAAAAGGCATCAAAGA-3′ |
MCM10 R
|
5′-GGAGTTTCCTTCCTGTGCTCT-3′ |
RPA70C F
|
5′-GATTACCTGCAGCAAACCAAG-3′ |
RPA70C R
|
5′-ACTCATAAGGGTTGGGCAGTT-3′ |
RPA70D F
|
5′-TGGATTTCGTCATTCAACGAT-3′ |
RPA70D R
|
5′-TAACCCGGAAGACATGAGATG-3′ |
N_UBQ5
|
5′-GGTGCTAAGAAGAGGAAGAAG-3′ |
C_UBQ5
|
5′-CTCCTTCTTTCTGGTAAACGT-3′ |
Primers for plasmid construction |
|
FHY3cdsF
|
5′-CACCATGGATATAGATCTTCGACTAC-3′ |
FHY3cdsR
|
5′-CGAGTGTCTAGACGCGTCCTCATGC-3′ |
SEP2cdsF
|
5′-CACCATGGGAAGAGGAAGAGTAGAGCTC-3′ |
SEP2cdsR
|
5′-TCACAGCATCCAGCCAGGGATGTAG-3′ |
SEP1cdsF
|
5′-CACCATGGGAAGAGGAAGAGTAGAGCTG-3′ |
SEP1cdsR
|
5′-TCAGAGCATCCACCCCGGGATGT-3′ |
SEP2cds2F
|
5′-CGGATCCATGGGAAGAGGAAGAGTAGAGCTC-3′ |
SEP2cds2R
|
5′-CCGCTCGAGTCACAGCATCCAGCCAGGGATGTAG-3′ |
SEP3proF
|
5′-GCACATCATCTCGATCAATTGTC-3′ |
SEP3proR
|
5′-CCTCTCCAGTGTCCGAAGCAT-3′ |
amiR-sep2AI
|
5′-gaTTCTAGTTGACGCTCAAGCTAtctctcttttgtattcc-3′ |
amiR-sep2AII
|
5′-gaTAGCTTGAGCGTCAACTAGAAtcaaagagaatcaatga-3′ |
amiR-sep2AIII
|
5′-gaTAACTTGAGCGTCTACTAGATtcacaggtcgtgatatg-3′ |
amiR-sep2AIV
|
5′-gaATCTAGTAGACGCTCAAGTTAtctacatatatattcct-3′ |
amiR-sep2BI
|
5′-gaTTATACTGTGTCTTGATGCAGtctctcttttgtattcc-3′ |
amiR-sep2BII
|
5′-gaCTGCATCAAGACACAGTATAAtcaaagagaatcaatga-3′ |
amiR-sep2BIII
|
5′-gaCTACATCAAGACAGAGTATATtcacaggtcgtgatatg-3′ |
amiR-sep2BIV
|
5′-gaATATACTCTGTCTTGATGTAGtctacatatatattcct-3′ |
Primers for mutant genotyping |
|
ag-10gtF
|
5′-CAATGTCTCCCAAAAGAGCCCAGGAACTT-3′ |
ag-10gtF
|
5′-GCAACAAGGCATATAGATTTAATTTG-3′ |
fhy3-68F
|
5′-TTTTGAAAACAACCAAGACCCAATGGTAACAT-3′ |
fhy3-68R
|
5′-CCGCAGCAGCCTAGTCTGCAACTG-3′ |
wus-1gtF
|
5′-AGTAGCCATGTCTATGGATCCATG-3′ |
wus-1gtR
|
5′-CCTCCACCTACGTTGTTGTAATTC-3′ |
clv3-1gtF |
5′-TGATGAAAATGGAAAGTGAA-3′ |
clv3-1gtR |
5′-AAAACCGATAAAGCAGAAAG-3′ |
fhy3-4gtF |
5′-GAAGCCAAAGCTGATCCAGAAATGT-3′ |
fhy3-4gtR |
5′-GTGGCATCTCGGTTCTCC-3′ |