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. 2016 Jul 25;113(33):E4837–E4846. doi: 10.1073/pnas.1601958113

Fig. 8.

Fig. 8.

Increased airway reactivity in allergen-challenged Gal-1–deficient mice. (A) Quantitation of the number of lung blood vessels with eosinophils (MBP-positive cells) and the number of adherent eosinophils in the blood vessels of OVA-challenged WT and Lgals1−/− mice. (B) Pulmonary resistance in saline- and OVA-challenged WT and Lgals1−/− mice following exposure to aerosolized methacholine. (C and D) Quantitation of mucus secretion (stained pink) in airways of saline- and OVA-challenged WT and Lgals1−/− mice along with representative images. BML, basement membrane length; PAS, periodic acid–Schiff reagent. (Scale bar, 50 µm.) (E and F) Quantitation of airway smooth muscle mass based on α-SMA IHC (stained brown). Representative images are shown in F. (Scale bar, 50 µm.) Combined data (mean ± SEM) of mice from two independent experiments (n = 7–8 mice per group) in A and three independent experiments (n = 7–8 mice for OVA groups and n = 6–7 mice for saline groups) in B, C, and E are shown. *P < 0.025 in A and *P < 0.05 at 6 and 25 mg/mL and *P < 0.03 at 12 mg/mL methacholine in B for comparison of OVA-challenged groups.