Fig. 2.

Seizure monitoring in vivo. (A) Experimental procedure. Juvenile mice (P18–25) were lightly anesthetized with urethane, and body temperature was maintained at 37 °C. Cortical EEG was recorded from an Ag/AgCl electrode implanted on the skull over the somatosensory cortex. Seizures were evoked by topical application of 4AP (100 mM). Heart rate was monitored with s.c. electrodes implanted on the thoracic wall. (B) Plot of survival rate following 4AP application. Seventy-one percent of RQ mutant mice (5 of 7) died shortly after 4AP-induced seizure, whereas none (0 of 7) of the WT mice died. (C) Representative recording showing terminal brainstem SD and cardiac arrhythmias following recurrent cortical seizures. Insets show EEG/EKG activity during baseline, during seizure (a), and after brainstem SD (b).